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AP Bio pGLO Transformation Formal Lab Report Essay Sample. The topic of war 1812 questions, this research involved the occurrence of genetic transformation in bacteria (E. Coli). More specifically, a previously prepared pGLO plasmidwhich consisted of the gene to project essay, be clonedwas used to war 1812 questions, transform non-pathogenic bacteria. The pGLO plasmid contained a gene for the Green Fluorescent Protein (GFP) from a bioluminescent jellyfish and a gene for duke, resistance to war 1812 essay, ampicillin, an antibiotic.
Essentially, we wanted to determine the conditions of the bacteria that would glow. Our hypothesis was that the transformed solution with no plasmid DNA and ampicillin would produce no bacteria colonies, as it wouldn#8217;t be able to manger resume, grow without the gene for ampicillin resistance. Also, the transformed solution with just LB and ampicillin would produce bacteria colonies but the transformed solution with LB/ampicillin/Arabinose would produce glowing bacteria colonies (as Arabinose allows the GFP gene to be expressed, but in both cases bacteria colonies would be present because of the essay gene of resistance to the antibiotic, ampicillin). We essentially made the required transformed solutionsand the controlsswiped them on the agar plate, and then observed to see whether or not bacteria colonies grew and whether or not they glowed. Our data fully supported our hypothesis. We can thus conclude that bacteria can take in foreign DNA through the process of transformation and behavior project essay that this foreign DNA can fundamentally change the bacteria (ex: making it glow). Questions. Future research can involve inserting other pieces of DNA into south park essays, bacteria from different organisms, making the bacteria take on various phenotypic characteristics. Genetic transformation is one of the most important processes in biotechnology. Essentially, genetic transformation involves the process where a cell (in this lab, a bacterial cell) takes up foreign DNA from its surroundings and incorporates it into its own DNA. This gene transfer is accomplished with the aid of a plasmid, a naturally-occuring small piece of circular DNA in war 1812 questions, bacteria.
Bacteria can efficiently adapt to new environments by transferring plasmids with beneficial genes aiding survival to other bacterial cells. In biotechnology laboratories, plasmids are transformed. Essentially, restriction enzymes are used to make cuts in the plasmidthe #8216;vector#8217;and in essay, the DNA sequence containing the gene to be transferred to the bacteria. Then, this gene to be transferred is forged into the plasmid (in this lab we used previously made plasmids called pGLO, containing a bioluminescent gene and a gene for ampicillin resistance). Plasmids are then #8216;forced#8217; into essay questions, bacterial cells using a process called #8220;heat shock,#8221; where small pores in the bacteria open up. In the manger resume endin order to find out which bacteria received the new genethe transformed solution can be incubated on agar gel. The transformed bacteria will exhibit certain unique characteristics (in our case actually growing, or glowing if Arabinose was present). Materials and essay Methods: For this experiment, the following materials were used: two micro test tubes, sterile transfer pipettes, 500 µl of Transformation Solution (CaCl2), an ice bath, a foam rack for the tubes to keep them afloat in the ice-bath, non-pathogenic E. Coli bacteria, sterile loops, pGLO DNA solution, four specially-prepared agar plates, LB-broth, and a water bath set at 42 degrees Celsius.
First we labeled one micro test tube #8216;+DNA#8217; and modification essay the other #8216;-DNA.#8217; Then we transferred 250 µl of Transformation Solution (CaCl2)using a sterile pipetteinto each micro test tube, and essay placed the sales manger tubes in a foam rack, which floated on an icy water bath. Next, we used two sterile loops to transfer E. Coli. Bacteria from the starter plate to each of the war 1812 tubes (#8216;+DNA#8217; and #8216;-DNA#8217; tubes). Now that we had added the required bacteria, we proceeded in our experiment by behavior modification essay adding the pGLO DNA solutionusing a new sterile loopto the micro test tube labeled #8216;+DNA.#8217; The next part of the experiment involved the heat shock process, where plasmids were fused into bacteria. We incubated both micro test tubes on ice for 10 minutes. Then, we transferred both of them into the water bath set at.
42 ?C for exactly 50 seconds, making sure to place them back on ice after the war 1812 essay questions 50 seconds were #8216;up.#8217; After letting the tubes incubate on ice for 2 minutes, we used a sterile pipette to add 250 µl of LB broth to the #8216;+DNA#8217; tube. We repeated this process with the #8216;-DNA#8217; tube. We let these tubes sit for ten minutes at room temperature. Finally, each transformation solution was prepared. Using new sterile loops for south, each plate, we spread the suspensions evenly around the surface of the agar of questions, each of duke, their four respective plates (plates were labeled +DNA LB/amp ; +DNA LB/amp/ara ; -DNA LB/amp ; -DNA LB for war 1812, what they contained or didn#8217;t contain [Arabinose, ampicillin, LB]). Lastly, we put our plates in an incubator so bacteria could grow, and observed them the next day under UV light. The nature of save me a in college contest, this research involved fusing pGLO plasmids containing the GFP glowing gene and war 1812 essay questions a gene for ampicillin resistance into E. Coli bacteria, only some of which would take in this plasmid. The solutions having transformed bacteria DNA from the plasmid (+DNA) and sales manger resume the solutions where its bacteria did not receive plasmid DNA (-DNA) were spread on questions, agar plates; the plates were then observed in the hopes of essays, drawing important conclusions about gene transfer and transformation in bacteria. In the agar plate with LB/Amp, where the solution with plasmid DNA combined into bacteria was spread, transformed white bacteria colonies were present, but no glowing under UV light was observed.
In the agar plate with LB/Amp/Ara, where the solution with plasmid DNA combined into bacteria was spread, transformed white bacteria colonies were present that glowed bright green under UV light. In the agar plate with LB/Amp and -DNA, no bacteria were present. In the agar plate with just LB and war 1812 questions -DNA, a bacteria lawn was present (bacteria were everywhere, but they did not glow). Our experiment was designed to observe the genetic transformation of non-pathogenic bacteria (E. Coli) via pGLO plasmids fusing into save in college contest, the bacteria. From careful analysis of war 1812, our results, it becomes clear that our hypothesis was supported. Behavior Modification Essay. Originally, we hypothesized that the only bacteria that would glow would be the bacteria that had received the plasmid DNAthat had been transformedbut that had also been cultivated in essay, Arabinose.
Also, we predicted the thorndike solutions with just the bacterianot the plasmid DNA in addition to the bacteriawould NOT grow if ampicillin was present in the growth medium, but would grow if ampicillin was absent from the war 1812 essay questions growth medium (agar). Our results confirmed our hypothesis. Park. Clearly, when analyzing our experiment, our hypothesis was supported by our results because it makes sense scientifically. First of all, any bacteria growing on the #8216;+DNA#8217; plates must have been transformed, since the plates have ampicillin, an war 1812 questions, antibiotic. In being transformed, bacteria received the manger resume gene for ampicillin resistance from the pGLO plasmid.
Thus, the only ones that grew were the ones that had the ampicillin-resistance-gene (which they received from the plasmids). Although all transformed bacteria will grow, only the bacteria present in arabinose will glow under UV light. This Arabinose is a sugar which turns on the GFP glowing gene. Furthermore, even bacteria which had not received the plasmid genes (#8216;-DNA#8217;), and questions thus could not glow, obviously would grow on duke cross continent mba essays, a medium with LB, favoring the growth of bacteria. However, if ampicillin is present, non-transformed bacteria will not grow, as they would not have received the ampicillin-resistance-gene from the pGLO plasmids. From this lab, we can conclude that bacteriathrough the process of essay questions, transformationare able to take up foreign DNA (such as in the form of manger resume, a pGLO plasmid) and questions express it in phenotypic characteristics (such as glowing). We also learned that transformation is never fully effective; only cells that are competent enough are able to take up foreign DNA. This can be seen by the fact that the control (-DNA/LB) plate had a lawn of bacteria, whereas the ampicillin plates had small bacteria colonies (only transformed bacteria could grow).
Our lab results are subject to imperfections, such as the fact that the heat shock timing might not have been perfect. Howeveroverallour results seem to be accurate; they are substantiated by current scientific knowledge in biotechnology. Is this the perfect essay for you? Save time and sales order AP Bio pGLO Transformation Formal Lab Report. essay editing for only $13.9 per page. Top grades and war 1812 essay quality guaranteed! Relevant essay suggestions for AP Bio pGLO Transformation Formal Lab Report.
Conjugation is a natural occurring process that involves the transfer of DNA from resume one cell into another through a physical connection between the cells. In the war 1812 following experiment, two strains#8230; Normal flora are found in cross continent, specific areas of the war 1812 questions body and modification project essay often depends on environmental factors such as pH, oxygen concentration, amount of moisture present, etc. Some sites in which#8230; Microbiology: Viable Counts. War 1812 Essay. Total Viable Count is a quantitative idea about the presence of microorganisms such as bacteria, yeast and mold in a sample. It counts the number of colonies produced by a#8230; The purpose of this report is to duke, use different tests to discover what bacteria is the unknown. This is important because we need to war 1812 essay, know what test need to be#8230; Medicinal Microbiology Lab Report.
Many infectious diseases caused by infectious agents, including bacteria, fungi, viruses and parasites, have plagued human existence. Thus the identification of save spot in college contest, bacteria becomes all the more important in the search#8230; Microbiology lab report on Escherichia coli and war 1812 essay questions Staphylococcus aureus. In this lab experiment two different types of bacteria, Escherichia coli and Staphylococcus aureus, were grown singly and mixed on park essays, four different types of agar in order to observe the#8230;
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Sample Essay Responses and Rater Commentary for essay questions the Argument Task. The sample essays that follow were written in response to the prompt that appears below . The rater commentary that follows each sample essay explains how the response meets the criteria for that score. For a more complete understanding of the criteria for each score point, see the edward Analyze an Argument Scoring Guide. In surveys Mason City residents rank water sports (swimming, boating and fishing) among their favorite recreational activities. Questions. The Mason River flowing through the city is rarely used for essay contest real simple these pursuits, however, and the city park department devotes little of its budget to essay maintaining riverside recreational facilities. For years there have been complaints from residents about the quality of the river's water and the river's smell. In response, the state has recently announced plans to clean up Mason River. Use of the river for duke cross mba essays water sports is therefore sure to increase. The city government should for that reason devote more money in this year's budget to riverside recreational facilities. Write a response in which you examine the stated and/or unstated assumptions of the argument.
Be sure to explain how the war 1812 argument depends on behavior the assumptions and what the implications are if the assumptions prove unwarranted. Note: All responses are reproduced exactly as written, including errors, misspellings, etc., if any. While it may be true that the Mason City government ought to devote more money to essay riverside recreational facilities, this author's argument does not make a cogent case for increased resources based on river use. It is easy to understand why city residents would want a cleaner river, but this argument is rife with holes and resume, assumptions, and thus, not strong enough to lead to increased funding. Citing surveys of city residents, the author reports city resident's love of water sports. It is not clear, however, the scope and validity of that survey. For example, the war 1812 essay survey could have asked residents if they prefer using the save spot essay river for war 1812 questions water sports or would like to see a hydroelectric dam built, which may have swayed residents toward river sports. The sample may not have been representative of behavior modification project essay, city residents, asking only those residents who live upon the river. The survey may have been 10 pages long, with 2 questions dedicated to river sports. War 1812. We just do not know. Unless the survey is fully representative, valid, and reliable, it can not be used to in college essay contest effectively back the author's argument.
Additionally, the essay questions author implies that residents do not use the river for swimming, boating, and fishing, despite their professed interest, because the save spot in college essay water is war 1812 questions, polluted and sales manger, smelly. While a polluted, smelly river would likely cut down on river sports, a concrete connection between the resident's lack of river use and the river's current state is not effectively made. Though there have been complaints, we do not know if there have been numerous complaints from a wide range of people, or perhaps from one or two individuals who made numerous complaints. To strengthen his/her argument, the author would benefit from implementing a normed survey asking a wide range of war 1812 essay questions, residents why they do not currently use the river. Building upon me a spot in college essay, the implication that residents do not use the river due to the quality of the river's water and the smell, the author suggests that a river clean up will result in increased river usage. If the river's water quality and smell result from war 1812 problems which can be cleaned, this may be true. For example, if the decreased water quality and aroma is caused by pollution by factories along the river, this conceivably could be remedied. But if the quality and aroma results from the behavior essay natural mineral deposits in questions, the water or surrounding rock, this may not be true. Lessons Contest. There are some bodies of water which emit a strong smell of sulphur due to the geography of the area.
This is not something likely to be afffected by essay questions, a clean-up. Consequently, a river clean up may have no impact upon river usage. Regardless of whether the river's quality is able to be improved or not, the author does not effectively show a connection between water quality and river usage. A clean, beautiful, safe river often adds to a city's property values, leads to increased tourism and south park, revenue from essay questions those who come to take advantage of the river, and a better overall quality of life for residents. For these reasons, city government may decide to invest in improving riverside recreational facilities. However, this author's argument is not likely significantly persuade the city goverment to project essay allocate increased funding. Rater Commentary for Essay Response Score 6. This insightful response identifies important assumptions and thoroughly examines their implications. The essay shows that the proposal to spend more on riverside recreational facilities rests on three questionable assumptions, namely: that the war 1812 questions survey provides a reliable basis for contest real simple budget planning that the river’s pollution and odor are the only reasons for its limited recreational use that efforts to clean the water and remove the odor will be successful.
By showing that each assumption is highly suspect, this essay demonstrates the weakness of the entire argument. Questions. For example, paragraph 2 points out that the edward thorndike survey might not have used a representative sample, might have offered limited choices, and might have contained very few questions on war 1812 water sports. Paragraph 3 examines the tenuous connection between complaints and limited use of the river for sales recreation. Complaints about water quality and odor may be coming from only a few people and, even if such complaints are numerous, other completely different factors may be much more significant in reducing river usage. Finally, paragraph 4 explains that certain geologic features may prevent effective river clean-up.
Details such as these provide compelling support. In addition, careful organization ensures that each new point builds upon the previous ones. War 1812. For example, note the clear transitions at the beginning of paragraphs 3 and park essays, 4, as well as the logical sequence of sentences within paragraphs (specifically paragraph 4). Although this essay does contain minor errors, it still conveys ideas fluently. Note the effective word choices (e.g., rife with . War 1812 Essay. . Mba Essays. . assumptions and may have swayed residents). In addition, sentences are not merely varied; they also display skillful embedding of subordinate elements. Since this response offers cogent examination of the argument and conveys meaning skillfully, it earns a score of war 1812 essay questions, 6. The author of this proposal to manger resume increase the budget for Mason City riverside recreational facilities offers an interesting argument but to essay questions move forward on the proposal would definitely require more information and thought. While the correlations stated are logical and probable, there may be hidden factors that prevent the City from diverting resources to this project. For example, consider the survey rankings among Mason City residents.
The thought is that such high regard for water sports will translate into behavior modification essay, usage. But, survey responses can hardly be used as indicators of actual behavior. Many surveys conducted after the winter holidays reveal people who list exercise and weight loss as a top priority. Yet every profession does not equal a new gym membership. Even the wording of the survey results remain ambiguous and war 1812 questions, vague. While water sports may be among the edward dissertation residents' favorite activities, this allows for many other favorites. What remains unknown is the questions priorities of the general public. Do they favor these water sports above a softball field or soccer field? Are they willing to sacrifice the municipal golf course for behavior project better riverside facilities?
Indeed the survey hardly provides enough information to discern future use of improved facilities. Closely linked to the surveys is the bold assumption that a cleaner river will result in increased usage. War 1812. While it is not illogical to expect some increase, at what level will people begin to use the river? The answer to this question requires a survey to find out the reasons our residents use or do not use the river. Is river water quality the primary limiting factor to usage or the lack of docks and edward, piers? Are people more interested in water sports than the recreational activities that they are already engaged in? These questions will help the city government forecast how much river usage will increase and to assign a proportional increase to the budget. Likewise, the author is optimistic regarding the state promise to clean the river. We need to hear the source of the voices and consider any ulterior motives. Is this a campaign year and the plans a campaign promise from the state representative?
What is the war 1812 essay timeline for the clean-up effort? Will the state fully fund this project? We can imagine the misuse of resume, funds in renovating the riverside facilities only to watch the new buildings fall into war 1812 essay questions, dilapidation while the state drags the river clean-up. Last, the duke author does not consider where these additional funds will be diverted from. The current budget situation must be assessed to determine if this increase can be afforded. Questions. In a sense, the essay City may not be willing to draw money away from other key projects from road improvements to schools and education. The author naively assumes that the money can simply appear without forethought on where it will come from. Examining all the various angles and factors involved with improving riverside recreational facilities, the argument does not justify increasing the budget.
While the proposal does highlight a possibility, more information is required to warrant any action. Rater Commentary for Essay Response Score 5. Each paragraph in the body of this perceptive essay identifies and examines an unstated assumption that is crucial to essay the argument. Cross Continent. The major assumptions discussed are: that a survey can accurately predict behavior that cleaning the river will, in itself, increase recreational usage that state plans to clean the river will actually be realized that Mason City can afford to spend more on riverside recreational facilities. Support within each paragraph is both thoughtful and thorough. For example, paragraph 2 points out vagueness in the wording of the survey: Even if water sports rank among the favorite recreational activities of Mason City residents, other sports may still be much more popular. Thus, if the first assumption proves unwarranted, the argument to essay fund riverside facilities rather than soccer fields or golf courses becomes much weaker. Paragraph 4 considers several reasons why river clean-up plans may not be successful (the plans may be nothing more than campaign promises or funding may not be adequate).
Thus, the weakness of the third assumption undermines the argument that river recreation will increase and riverside improvements will be needed at all. Instead of lessons simple, dismissing each assumption in isolation, this response places them in a logical order and considers their connections. Note the appropriate transitions between and within paragraphs, clarifying the links among the assumptions (e.g., Closely linked to war 1812 essay questions the surveys or The answer to this question requires. ). Along with strong development, this response also displays facility with language. Minor errors in edward dissertation, punctuation are present, but word choices are apt and sentences suitably varied in pattern and length. The response uses a number of rhetorical questions, but the implied answers are always clear enough to support the points being made. Thus, the essay questions response satisfies all requirements for a score of 5, but its development is save me a in college essay contest, not thorough or compelling enough for a 6. The problem with the arguement is the assumption that if the Mason River were cleaned up, that people would use it for water sports and essay questions, recreation. This is not necessarily true, as people may rank water sports among their favorite recreational activities, but that does not mean that those same people have the financial ability, time or equipment to pursue those interests.
However, even if the writer of the arguement is correct in assuming that the Mason River will be used more by essay, the city's residents, the arguement does not say why the recreational facilities need more money. If recreational facilities already exist along the Mason River, why should the city allot more money to fund them? If the questions recreational facilities already in existence will be used more in the coming years, then they will be making more money for themselves, eliminating the edward need for the city government to devote more money to them. According to questions the arguement, the reason people are not using the Mason River for water sports is because of the smell and the quality of life essay simple, water, not because the recreational facilities are unacceptable. If the city government alloted more money to the recreational facilities, then the budget is being cut from some other important city project. Questions. Also, if the assumptions proved unwarranted, and more people did not use the river for recreation, then much money has been wasted, not only the money for the recreational facilities, but also the me a in college essay money that was used to essay questions clean up the river to attract more people in the first place.
Rater Commentary for edward thorndike dissertation Essay Response Score 4. This competent response identifies two unstated assumptions: that cleaning up the Mason River will lead to increased recreational use that existing facilities along the questions river need more funding. Paragraph 1 offers reasons why the first assumption is questionable (e.g., residents may not have the necessary time or money for water sports). Similarly, paragraphs 2 and manger, 3 explain that riverside recreational facilities may already be adequate and war 1812, may, in fact, produce additional income if usage increases. Park Essays. Thus, the response is adequately developed and satisfactorily organized to show how the argument depends on questionable assumptions. However, this essay does not rise to war 1812 essay questions a score of 5 because it fails to consider several other unstated assumptions (e.g., that the south essays survey is reliable or that the efforts to clean the war 1812 questions river will be successful). Furthermore, the final paragraph makes some extraneous, unsupported assertions of its own. Mason City may actually have a budget surplus so that cuts to thorndike other projects will not be necessary, and cleaning the river may provide other real benefits even if it is not used more for water sports. This response is generally free of errors in grammar and usage and displays sufficient control of essay questions, language to support a score of 4. Surveys are created to speak for manger the people; however, surveys do not always speak for questions the whole community. A survey completed by Mason City residents concluded that the modification essay residents enjoy water sports as a form of recreation.
If that is so evident, why has the river not been used? The blame can not be soley be placed on the city park department. The city park department can only do as much as they observe. The real issue is not the residents use of the river, but their desire for a more pleasant smell and a more pleasant sight. If the city government cleans the river, it might take years for the smell to go away. If the budget is changed to war 1812 questions accomodate the clean up of the Mason River, other problems will arise. The residents will then begin to south essays complain about other issues in their city that will be ignored because of the great emphasis being placed on Mason River. If more money is taken out of the budget to clean the war 1812 essay questions river an assumption can be made. This assumption is life essay real simple, that the budget for another part of cit maintenance or building will be tapped into to. In addition, to the budget being used to clean up Mason River, it will also be allocated in increasing riverside recreational facilites.
The government is trying to appease its residents, and one can warrant that the role of the government is to please the war 1812 essay questions people. There are many assumptions being made; however, the government can not make the assumption that people want the river to essays be cleaned so that they can use it for recreational water activities. The government has to realize the long term effects that their decision will have on the monetary value of their budget. Rater Commentary for Essay Response Score 3. Even though much of this essay is tangential, it offers some relevant examination of the essay argument’s assumptions. The early sentences mention a questionable assumption (that the manger survey results are reliable) but do not explain how the survey might have been flawed.
Then the response drifts to irrelevant matters a defense of the war 1812 essay questions city park department, a prediction of budget problems and the problem of pleasing city residents. Some statements even introduce unwarranted assumptions that are not part of the original argument (e.g., The residents will then begin to complain about essay real simple, other issues and This assumption is that the budget for another part of essay, city maintenance or building will be tapped into). Near the end, the behavior essay response does correctly note that city government should not assume that residents want to use the river for recreation. Hence, the proposal to increase funding for war 1812 questions riverside recreational facilities may not be justified. In summary, the language in this response is reasonably clear, but its examination of unstated assumptions remains limited and therefore earns a score of 3. This statement looks like logical, but there are some wrong sentences in it which is edward thorndike dissertation, not logical. First, this statement mentions raking water sports as their favorite recreational activities at essay, the first sentence.
However, it seems to have a ralation between the cross first sentence and the setence which mentions that increase the quality of the river's water and the river's smell. This is a wrong cause and war 1812, result to solve the problem. Second, as a reponse to edward thorndike the complaints from residents, the state plan to clean up the river. As a result, the essay state expects that water sports will increase. When you look at two sentences, the dissertation result is not appropriate for the cause. Third, the last statement is the conclusion. However, even though residents rank water sports, the city government might devote the budget to another issue. War 1812 Essay. This statement is also a wrong cause and essays, result.
In summary, the statement is not logical because there are some errors in it. The supporting setences are not strong enough to support this issue. Rater Commentary for Essay Response Score 2. Although this essay appears to be carefully organized, it does not follow the directions for the assigned task. War 1812 Essay. In his/her vague references to causal fallacies, the writer attempts logical analysis but never refers to any unstated assumptions. Furthermore, several errors in grammar and sentence structure interfere with meaning (e.g., This statement looks like logical, but there are some wrong sentences in it which is not logical). Because this response does not follow the directions for the assigned task and contains errors in sentence structure and logical development, it earns a score of 2. The statement assumes that everyone in Mason City enjoys some sort of thorndike, recreational activity, which may not be necessarily true.
The statement also assumes that if the state cleans up the river, the use of the war 1812 essay questions river for edward thorndike water sports will definitely increase. Rater Commentary for Essay Response Score 1. The brevity of this two-sentence response makes it fundamentally deficient. Sentence 1 states an assumption that is actually not present in the argument, and sentence 2 correctly states an assumption but provides no discussion of its implications. Although the response may begin to address the assigned task, it offers no development.
As such, it is clearly extremely brief . providing little evidence of an organized response and earns a score of 1. Register for the GRE General Test. Get never-before-offered official GRE practice tests, scores within minutes for essay all three measures and explanations for correct answers! Show schools only the scores you want them to behavior project see only with the ScoreSelect ® option. Registration, Test Centers and Dates ¡ Registration, Test Centers and Dates ¡ ETS Measuring the war 1812 essay questions Power of Learning. ® Copyright 2017 by dissertation, Educational Testing Service.
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The No BS Guide to Building a Powerful Small Business Web Presence. You’re a new small business owner that wants to build a brand and web presence on the internet — but you’re overwhelmed with all of the information that’s scattered across sites online. That’s why I’ve decided to create this practical guide that covers the basics that you will need to get started with building an impactful digital presence for questions, your small business that makes it easy for customers to find you + access information about your business. An online presence is essentially made up of all of the platforms + brand that you establish online (ie., via a website, blog, or social media networks) These platforms are used to help you get your business out cross mba essays there to the marketplace and make your business more accessible to customers via the web. Why Building a Small Business Web Presence Matters.
Well for war 1812 questions, starters, 85% of edward thorndike, consumers (aka your clients and prospects) are online looking for essay, products and services that you offer. So there’s no better time like the present to mba essays establish a “ Digi Home ” for essay questions, your business as quoted by one of my favorite bloggers and entrepreneurs, Nikki Elledge Brown. Oh and life lessons essay contest here are some additional interesting stats that you should consider: The 2012 Digital Influence Index Shows Internet as Leading Influence in Consumer Purchasing Choices ( Fleishman-Hillard ) 89 percent of consumers use search engines for purchase decisions ( Fleishman-Hillard ) 94% of smartphone owners look for local information on essay questions their phone ( Televox ) 79 percent of behavior modification project, consumers said they Like or follow brands on social networks to war 1812 questions get more information about the company and essay simple its products and service ( Fleishman-Hillard ) A Little Bit of questions, My Backstory. I got started with building a digi presence about 2 years ago…and man was I like a fish out of water. Like many of you new to the online world, I was overwhelmed, frustrated and confused with trying to establish a home on the web. This process was pretty painful and resume VERY expensive. I spent a lotttt of money on stuff that (frankly I didn’t need) and on war 1812 essay questions programs that didn’t deliver on their promises, worthless tech tools and etc.,
The short of the story is this: It took me two years to really understand how all of this stuff works. But here’s the thing: Everything that I’ve experienced has been worthwhile. It has brought me to a place where I can share information with you all that will help you easily get your small business setup online — faster than I was able to. How to dissertation Create a Web Presence For Your Small Business. 1. Establish clear goals for questions, getting setup online . Before you start setting up shop online you will need to establish some clear goals for your business. You will need to make sure that your online activity aligns with your business goals.
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Setting up a website or blog is a super essential requirement for creating an impactful online presence for your small business. A website is a 24/7 shop that showcases your products or services to essay the world. It is one of the south, best investments that you can make in your small biz that will make your business accessible online to customers. One of the most popular platforms that you can use to easily create a website for your small biz is WordPress . If you’re not tech savvy, Squarespace is an additional platform available that you can use to create a professional looking website on the cheap as well. A blog is an additional digital platform that you can use to showcase your services and get your business in front of more people online. In fact, more people are using blogs to essay questions establish a business presence via the life lessons contest simple, web. Blogs can be started in a matter of minutes and offer an easy way to communicate with the audience that you want to reach. The biggest thing that you need to remember when starting your blog is to keep your blog content fresh and war 1812 questions updated regularly. Here again, WordPress is the most popular blogging platform used by the pros.
4. Sign up for a Web Hosting Account. A web hosting provider is the company that you will use to manger resume host your website or blog — and make it accessible to the world online. Two of my favorite and reliable web hosting providers are Hostgator and Siteground . Design matters peeps! I cannot stress this enough. Think about war 1812 questions, it this way, when you visit a site that looks a tad bit sketchy what do you do?
Probably navigate away from the site right… Fortunately for you, sites like Themeforest and Creative Market have themes that you can purchase to setup a digi home for your small biz on the cheap. Your brand statement should identify who you serve and what you help them do. Your brands message is me a in college contest, essential in the online marketplace. Questions. A core message will helps your prospects identify with you and if they are in me a spot in college the right place once they land on war 1812 essay your website or blog. Twitter is an excellent resource that you can use to life lessons simple look at examples of brand statements from war 1812 essay different brands in your industry and also get ideas on how to craft a brand statement and edward dissertation message for war 1812 essay questions, your small business. Social media is life lessons essay, a POWERFUL communications channel for getting your business in front of more prospects and customers via the war 1812 questions, web easily and at a fraction of the sales resume, cost of traditional marketing and questions advertisement services. Twitter and Facebook are the two of the main social media platforms that I am using to promote this brand. If you’re just starting out, you should consider the south park essays, following: Which platform has the greatest percentage of your ideal audience active on there What kind of content you will need to create to attract them to your business/brand Are there tools available that you can use to manage the social media account easier? 8 . Get a professional photo/headshot taken . Add it to your site, blog, social media profiles, email signature and etc.
I didn’t realize how BIG of a difference this can make but it does. A professional photo makes for a positive impression online and inspires trust from the folks that come across your biz via the web. If you can’t afford to hire a professional photographer don’t worry. Smartphone apps like Camera Zoom FX for Android smartphones and Camera+ for iPhones will help you take great photos on a budget. I used my iPhone to take the pic below. To build your audience, it’s great to get in the habit of essay questions, posting content to your social media accounts. Posting content will attract new visitors to your business and behavior modification will keep your fans engaged. To save time, use Buffer and Hootsuite to pre-schedule + automate content so that you don’t have to war 1812 questions manually go back and forth between accounts posting content.
Let’s face it… content is duke continent mba essays, king. Writing great content, then marketing it online is war 1812, one of the best ways to duke continent attract the right people to you. The key is to write posts that speak to war 1812 your target audience, solves a problem for them and compels them to take action. Quick Recap of What We’ve Covered + Your Action Plan. Okay so here is a quick rundown and action plan for you to follow to build a presence online for your small biz: Establish clear goals for lessons essay, setting up shop online Purchase a memorable domain Setup a blog or website Sign up for a Web Hosting Account Invest in a professional theme (aka template) for your digital home Create a memorable brand statement and message. Setup accounts on social media platforms Get a professional photo taken Create share worthy blog posts (not me too content) Publish your content to your social media accounts regularly. Alrighty…over to war 1812 you good people. What are you doing to build an online presence for your small business? Leave your comments below or drop me a line on Twitter to let me know what’s working for you. As they say, sharing is caring.
** Post contains affiliate links for your convenience. BONUS GUIDE: Want a WORKBOOK that you can use track your online progress? Enter your name and email address below to download my FREE 10 Step Blueprint and Workbook to creating a POWERFUL Online Presence for your small business. GET YOUR FREE WORKBOOK TO BUILDING AN ONLINE. Presence that Sweeps your Audience Off Their Feet! Enter your name + email address to edward dissertation get instant access to war 1812 the workbook to building an online presence that sweeps your audience off their feet! 5 Emails to Add to Your Autoresponder Sequence. 5 Features that Made Me Fall Hopelessly in Love with Active Campaign. One Incredibly Easy Way to Streamline your Blog’s Design. 15 Fantastic Resources for Creating Killer Blog Post Titles.
Kim, YourChicGeek is a tech savvy diva + founder of YourChicGeek.com. Modification Project Essay. She is essay, passionate about teaching small biz's how to essay go from unnoticed to unstoppable online using tech and digital marketing. Kim has created several online courses that help small businesses flourish online. You can contact Kim to hop on a quick 15 minute call to chat about your digital marketing goals. Thanks for presenting these steps in the way that you did.
It a nice, simple idea to summarise the post by listing the ideas at war 1812 the end. Having a good web presence certainly matters. Hello there Nathan! Thank you sooo much for taking the time to drop by the blog and comment on this post. Save Me A Spot. Having a good web presence (especially these days) is very important for biz’s + bloggers alike. I am glad that you appreciated the essay questions, way that I outlined the thorndike dissertation, action items towards the end. Your support and retweets are very much appreciated. What a great post and I had to questions come back around to spot contest comment. I know what you mean about it taking you two years to learn what you should do all while most of essay questions, what you purchased along the sales, way didn’t quite deliver.
People don’t understand everything that’s involved in getting your presence up and war 1812 essay running so anytime they can learn from someone who has done the work and hopefully help them save a heck of life lessons contest simple, a lot of time then they should jump on that. Hope anyone who is at that beginning will devour this post and then go to work. Appreciate you sharing it and happy holidays! Well hello there Adrienne! Wow, thank you so much for your comments on this post. I was just thinking about your statement regarding purchasing programs + etc, that dont quite live up to the hype. War 1812. I have been there, done that and have gotten a T-shirt for it (as my mom says).
There is no magic formula or magic tricks that you can use to build up a solid presence via the web. I want to make sure that readers understand that it takes a lot of south park essays, time and effort to get things going, but once you do it is so worth it. Wishing you a very Happy Holiday season, and questions thank you again for continent, taking the time to essay drop by to edward dissertation comment. War 1812 Essay. Here’s to an Awesome 2016 #128578; I’m glad you covered this topic because the lack of a real web presence is lessons, a big issue I see so many small businesses in essay questions my community going through. To many of them, having a web presence is little more than a social media page they will post to once a week or so. It’s kind of sad.
The fact of the matter is as business owners we need to go out and become part of edward thorndike dissertation, our target market’s lives, we need them thinking about us. With more and more people spending more of war 1812, their time online, how can we expect to essays do that without a web presence right? No doubt this is going to help a lot of people struggling in this area. Love the site by questions the way. I know there’s going to be a lot of great stuff coming from manger resume you in essay the future, so I’m really looking forward to it! Hello thereeee James! I am soo glad that you dropped by to take the time to essay contest real simple comment on war 1812 questions this topic. As you can see, this is one that is near and south park dear to my heart, especially after trying to figure out how to get set up on essay questions the web properly. As you stated, in dissertation this day in time, a Facebook page is just not going to cut it..because as you stated, people are turning to the web to look for information..and if you are not making your business accessible online the right way, then its hard for war 1812 questions, your target customers to find you.
Again, Thanks so much for dropping by. Wishing you a blessed and duke cross prosperous New Year #128578; This was a great break down of what you need to focus on to succeed online. As you mentioned from your experience internet marketing can be confusing and overwhelmed with information for a newbie. War 1812 Essay. But a guide like this will definitely help to stay on track with what you have to. Thanks Kim for sharing and Happy Belated New Years! Hello there Sherman! I am sooo sorry that I am late to the party my friend.
Thank you so much for dropping by to comment on the blog post. Yes, for a newbie that is just getting started online, it is life essay real, VERY overwhelming. Truth is…these are things that I wish I would have known years go. I am glad that you liked the essay questions, post my friend. Cheers and heres to an AWESOME 2016. My first visit to your place and what a refreshingly helpful post. I can truly relate to your getting set up online challenges – I probably wasted way too much time, money and modification emotional energy chasing all sorts of “shiny objectives” that promised me all sorts of amazing results…that never eventuated!
I have shared this post widely and essay questions will also give a link to it to my list as your 10 point checklist is something I feel that anyone new online (or even considering that option) should read, learn and then take considered action on. The idea of having clear goals especially when it somes to park your target audience is something that I know many folks struggle with. It’s just so important to really nail down exactly who you are trying to essay questions reach and then work out where they hang out online. When you have worked out who your target is and where they are online then the next logical step is to discover what they want….and simply give it to essays them. People need to get these 3 steps correct before implementing the rest of war 1812 essay questions, your excellent 10 point blueprint. Many thanks for sharing this superb guide Kim. Best wishes from a remote Thai village entrepreneur. Heyyyy there Peter!
Wowza. South Essays. Thank you sooo much for essay, stopping by to comment. It is true my friend, setting up online can be a little taxing at first, especially if you are new to the online world. When I first started out, my biggest issue was figuring out who I was targeting. Fortunately, but unfortunately, I spent years blogging on a previous site trying to speak to in college essay EVERYONE. As they say in Marketing, when you are talking to everyone…you are talking to no one. With the help of coaching by a good friend Don Purdum, I was finally able to nail down a target market and put a solid strategy and plan of action in place to reach them. Glad that you enjoyed the war 1812 essay, post my friend. Cheers and best wishes to you this year #128578;
You actually just hit the edward, nail right on war 1812 the head in your comment and its something that many online marketers fail to cross continent understand and accept. We all need a coach and to stop fooling ourselves that we can do this business all on essay our own. It took me some time to get to this conclusion as probably my ego was in the way! But as soon as my mindset accepted that I needed help and guidance my online presence and business took off. Thanks for sharing and cross mba essays I look forward with great anticipation to your next post creation. Peter…that is awesome news my friend and yes you are right! I tried for 2 years to do everything on my own and figure things out….(BIG Mistake) Hiring Don was absolutely + hands down one of the best investments and decisions that I have made for my business. Again, thank you for dropping by.
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A Good Deed Indeed Essay Essays and Research Papers. Everyman, a morality play, utilizes ten characters to questions guide Everyman on his journey towards death. Through the plot Everyman is thorndike, chosen by god as an example . to all humans. God feels unappreciated and is not happy with human obsession over material goods . Plot is used as a device to place events in sequence to make up a theme. Cause and effect is a tool of plot used in Everyman. The author uses Fictional and allegorical characters to help the protagonist learn a lesson. The author’s use of essay, allegory. Character , Climax , English-language films 1442 Words | 4 Pages. Author’s Perception of Death “The medieval morality play Everyman, personifying such abstractions as Fellowship and park Good . Deeds , recounts the death journey of Everyman” (Allegory, 2010). War 1812 Essay Questions? The author uses symbolic names for characters to emphasize the moral of the play. “The characters in an allegory often have no individual personality, but are embodiments of resume, moral qualities and other abstractions” (Allegory, 2010) The author sees death as important as life, especially when death comes to makes. Christian terms , Death , Heaven 698 Words | 3 Pages.
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Everyman’s Good Deeds – for Life or Death? `Everyman’s Good Deeds – For Life or Death? Everyman, in its attempt to clearly depict the importance of man’s morality, . Essay? focuses on a faith based on works, however; this focus is not on good deeds already obtained but on locating said deeds before proceeding to death. It would seem, then, that it is not necessary for Everyman to reflect on good deeds he has performed but that he find a way to acquire them quickly. Unlike the Protestant view, which bases religion on faith alone, Everyman. Black-and-white films , Debut albums , Form of the Good 1196 Words | 3 Pages. ? No Good Deed Goes Unpunished A Case Study In Partial Fulfillment of MFC301 Ethical Foundation . of Enterprise Management Submitted by Carmelita Nova M. Alcantara I. Problem Corporate citizenship, an project essay, advocacy of companies to maintain their business activity to war 1812 essay questions act and behave responsibly in thorndike respect to war 1812 questions environment for the common good . Life Essay Contest? Despite the essay, nobility of its goal however, some critics have regarded this advocacy. Modification? Business , Business ethics , Corporate behaviour 2178 Words | 7 Pages. 15 Easy Good Deeds to Do Throughout Ramadan. 12 sunnah rakat (as follows) 4 rakat before and 2 after Dhur, 2 after the Maghrib, 2 after Ishaa, and 2 before Fajr.” [Tirmidhi] 3. Attending lectures in . the Masjid. Questions? “Whoever goes to edward thorndike dissertation the mosque not desiring except to learn or teach what is good has the reward of a pilgrim who completed his Hajj” [Tabarani] 4. Visiting a sick Muslim. Essay Questions? .”“There is no Muslim who visits a sick muslim early in the morning but 70 thousand angles send blessings upon him until evening comes, and if he visits.
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Free Essays on Film Character Analysis. Writing Response: Whaler Rider (2002) In the film Whale Rider (2002) Nikki Carro takes us on a journey of a twelve-year-old Maori girl, Paikaea Apirana, (Pai), and questions, her attempt to fulfill her brothers destiny. Edward? Her story begins chaotic, during Pai’s birth her twin brother and war 1812 questions, mother died, leaving. ?Karla Galomo Film as Literature Ms. Lazzarini 28 Aug.
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